LMP1203H: Basic principles of analytical clinical biochemistry

Who can attend

To attend this course, you must have permission from your supervisor and be:

  • registered in our graduate program; or
  • be a medical or clinical biochemistry resident or fellow.

Course description

You will learn about modern analytical techniques and limitations as applied in clinical laboratories.

Topics include the basic theory and applications of analytical techniques that are commonly used in clinical laboratories.

Course coordinator

Dr. Pak-Cheung Chan

lmp.grad@utoronto.ca for administrative queries.

Timings and location

This course is offered every year in the Fall session.

Wednesdays, (9:00 a.m. - 12:00 noon)

Location: Synchronous online delivery (Zoom)

Evaluation methods

Home assignments and final end-of-course exam.


Date Topic Lecturer/instructor
September 2, 2020 Method evaluation, analytical & clinical Dr. Davor Brinc
September 9, 2020

General lab techniques: centrifugation, water purification and QA osmometry, radioactivity, viscometry,

dialysis, freeze drying, buffers, etc

Dr. Felix Leung
September 16, 2020

Enzymatic techniques and chemistry analyzers

Dr. Felix Leung
September 23, 2020

Co-oximetry, Biosensors, Nanotechnology and POC instruments 

Dr. Davor Brinc
September 30, 2020

Ion selective electrodes

Dr. Mohamed Abouelhassan
October 7, 2020 Electrophoresis and related techniques Dr. Pak-Cheung Chan
October 14, 2020 Chromatographic techniques: TLC, LC, GC, HPLC Dr. Daniel Beriault

October 23, 2020


Mass Spectrometry

Friday, not Wednesday.

Dr. Vathany Kulasingam
October 28, 2020 Immunoassays I: Properties of Antibodies and Antigens; Ag-Ab Interactions; Ab production Dr. Paul Yip
November 4, 2020

Immunoassays II: Configurations, Labels and Interferences 

Dr. Paul Yip
November 11, 2020

Immunoassays III: Fluorescence and Luminescence 

Dr. Pak-Cheung Chan
November 18, 2020

Spectrophotometric techniques: reflectance, absorbance, transmittance, turbidimetry and nephelometry; Flame emission and absorption spectrometry;

Infra-red, and NMR.

Dr. Maria Pasic
November 25, 2020

Misc Techniques 1: Molecular diagnostics

Dr. Cuihong Wei
December 2, 2020

Misc Techniques 2: Ultracentrifugation and lipid analyses

Dr. Dana Bailey


December 9, 2020 Final Examination (3 hours) Dr. Pak-Cheung Chan